Antibodies: A Laboratory Manual, Second edition

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Antibodies: A Laboratory Manual, Second edition

Edward A. Greenfield, Dana-Farber Cancer Institute

ISBN10: 1936113813

ISBN13: 9781936113811

Antibodies: A Laboratory Manual, Second edition

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It has been 25 years since the now classic lab manual Antibodies, by Harlow and Lane appeared. This second edition of Antibodies, edited by Edward A. Greenfield of the Dana-Farber Cancer Institute builds on the core strengths of the first edition, presenting clear and authoritative protocols with extensive background information and troubleshooting advice. The original introductory chapters have been recast and updated to take into account our current understanding of the immune system. The critical chapters on generating monoclonal antibodies and growing hybridomas, which demystified hybridoma generation, have been greatly expanded and updated to make these procedures easy to follow and adaptable to current research needs. The remaining chapters contain all new protocols and topics and reflect the progress in how antibodies are studied and used since the appearance of the original edition. These include:

  • antibody purification and storage
  • engineering antibodies, including use of degenerate oligonucleotides, 5'-RACE, phage display, and mutagenesis
  • extensive labeling techniques
  • new immunoblotting protocols
  • the latest screening and labeling techniques

As Dr. Greenfield notes in his preface to this second edition: "The Antibodies manual provided our laboratory with guidance in the form of protocols and recommendations for setting up a hybridoma facility. Everything we needed to know to make a monoclonal antibody was all there, neatly packaged in an easy-to-understand book....the second edition is intended to provide the necessary information and protocols to assist investigators with their first monoclonal antibody effort as well as to provide guidance for more experiences antibody makers who are having some difficulties with a particular project."

What's New in This Edition?

I have the first edition. Why should I buy the second edition?

While the core of the first edition appears in updated and recast form in the second edition, more than half of the second edition contains entirely new information and protocols to reflect the changes in the field since the first edition.

To whom is this edition addressed? Is this a book for immunologists?

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Book Table: Antibodies: A Laboratory Manual, Second edition:

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title Antibodies
subtitle A Laboratory Manual
authors Edward A. Greenfield
publisher Anchor Books
publishedDate 2013-09-30
description "The focus of Antibodies: A Laboratory Manual, 2nd Edition, will be unchanged from the original edition by Ed Harlow and David Lane and will cover both the production and use of antibodies in a way that is accessible to the nonimmunologist. The emphasis will be on contemporary, essential antibody-based methods that are tried, true, necessary, and useful to a broad population of life scientists. The manual will provide up-to-date protocols that work reproducibly, along with explanations as to how and why methods work and how to choose between alternative approaches. Methods that have become research staples since the manual was originally published will be included at the same level of detail and organization as the existing topics"--
pageCount 847
printType BOOK
categories Science
maturityRating NOT_MATURE
language en

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